The EliSpot technology is widely used to quantify antigen-specific cellular immunity on a single-cell level. The T-cell reaction against a specific antigen is the central part of the cellular immune answer. Individual immunity can be monitored in detail based on the number of activated T-cells and the secreted cytokine.
The ELISPOT assay is a well standardized method and easily automatable for different issues and questions and is therefore used in research as well as in diagnostic laboratories.
AID Classic is the classic EliSpot reader and is fast, efficient and user friendly. The AID Classic interprets any type of 96 and 384-well plates, including all brands of membrane type plates, ELISA-style plates and low volume plates. It simultaneously takes high resolution images, auto-centers the well, counts/analyzes plates and exports data in various formats.
AID iSpot allows the analysis of both enzymatic and fluorescent EliSpot assays.
In addition to the functionality of the AID Classic, the AID iSpot allows the analysis of 1-, 2- or even 3-color FluoroSpot assays. A simple “one-click switch” between enzymatic and fluorescent mode, without the need of hardware changes, is enough to switch from one mode to another. The “FluoroAID” image overlay technology permits exact detection of cells secreting multiple cytokines. Count results and all other parameters can be exported in various formats.